PTEN Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > PTEN Knockout cell line (Hep G2)

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PTEN Knockout cell line (Hep G2) Order

PTEN Knockout cell line (Hep G2)
Catalog#
YKO-HT1469
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTEN Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PTEN Knockout cell line (Hep G2)
Species
Human
Gene Name
PTEN
Gene ID
5728
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript PTEN-201)
Validation: PCR+Sanger Sequencing
PTEN Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTEN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTEN gene knockout PTEN Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTEN Knockout cell line (Hep G2)
PTEN Knockout cell line (Hep G2)
Catalog#
YKO-HT1469
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PTEN Knockout cell line (THP-1)

    PTEN Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-HT0082
    YKO-HT0082(THP-1)
  • PTEN Knockout cell line (HCT 116）

    PTEN Knockout cell line (HCT 116）
      inquiry   More details

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-H243
    YKO-H243(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PTEN Knockout cell line (THP-1)

    PTEN Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-HT0082
      inquiry   More details
  • PTEN Knockout cell line (HCT 116）

    PTEN Knockout cell line (HCT 116）

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-H243
      inquiry   More details

PTEN Knockout cell line (Hep G2) Order

PTEN Knockout cell line (Hep G2)
Catalog#
YKO-HT1469
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTEN Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PTEN Knockout cell line (Hep G2)
Species
Human
Gene Name
PTEN
Gene ID
5728
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript PTEN-201)
Validation: PCR+Sanger Sequencing
PTEN Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTEN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTEN gene knockout PTEN Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTEN Knockout cell line (Hep G2)
PTEN Knockout cell line (Hep G2)
Catalog#
YKO-HT1469
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PTEN Knockout cell line (THP-1)

    PTEN Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-HT0082
    YKO-HT0082(THP-1)
  • PTEN Knockout cell line (HCT 116）

    PTEN Knockout cell line (HCT 116）
      inquiry   More details

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-H243
    YKO-H243(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PTEN Knockout cell line (THP-1)

    PTEN Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-HT0082
      inquiry   More details
  • PTEN Knockout cell line (HCT 116）

    PTEN Knockout cell line (HCT 116）

    Organism: Human
    Gene Name: PTEN
    Gene ID: 5728
    Size: 1*10^6
    Catalog#: YKO-H243
      inquiry   More details

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